Avinash Kumar:
A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples -> https://docs.google.com/document/d/1kP2w_uTMSep2UxTCOnUhh1TMCjWvHEY0sUUpkJHPYV4/edit?usp=drivesdk
so the NGS devices given to BLS3 labs can help speed up testing like from IITs?
Rohit- Yes. The possible reason they weren't using it because of the cost involved in sequencing. The kits are usually expensive along with the reagents
A- but become useful when large (not very large like 19200 but something like 200-300) patient samples to be tested?
200*145$ = 29000$ so then NGS way will be cheap and accurate?
R- Can't comment on this protocol. But looks interesting. However, the picture is blurred and I can't really understand wetlab much
Where is this cost coming from Avi?
Are we using biological replicates in this protocol. If yes how many??
A- 145$ is lowest cost from latest testing available afaik, and 300$ is for normal one that is used now. its approx and none of above info i have verified personally, but believe to be true
R- Depends Avi how deeply u sequence
only few parts you didn't get or many?
A- how deep we need to confirm if its no covid with high confidence? this can be extremely useful to mass screen communities in batches and seize them to prevent much harm to the economy and there are good ways coming ahead to do that part
R- Okay so they are using 2 amplicons
And 1 control
In total 3
I can't comment on that avi. Haven't had experience handling sequencing data of a virua
A- ok so this info came for 145$ test I had found - Update: After this story was published, the U.S. Food and Drug Administration issued a warning that at-home coronavirus tests are unauthorized. Read story here. https://www.statnews.com/2020/03/20/at-home-coronavirus-tests-are-here-should-you-take-them/
the at lab tests are arond 300 usd and in india around 10k afaik (govt.asked pvt.labs to do at 4500 let see how many does)
https://twitter.com/koeppelmann/status/1245037948099330048
B- The report suggests use of this kit the cost of which is following
A- R and i discussed same above. seems possible.
R- Yes Avinash. I think it's possible
We adopt such approach in NGS
R- I though doubt if the binary testing will actually save testing kits though
the grouping must be done carefully,Like all the samples coming from a single family will be a good idea In case if say out of 16 samples 8 tests positive
it will not help when we are testing symptomatic, or high risk, but can help if we mass testing and saving them who are negative from infection. like even a family or 2, unless whole family is high risk or having anyone symptomatic
R- Then if these guys are using some kind of index primers that are patient specific then this can really scale up testing
16/16 or 0/16 are ideal cases
A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples -> https://docs.google.com/document/d/1kP2w_uTMSep2UxTCOnUhh1TMCjWvHEY0sUUpkJHPYV4/edit?usp=drivesdk
so the NGS devices given to BLS3 labs can help speed up testing like from IITs?
Rohit- Yes. The possible reason they weren't using it because of the cost involved in sequencing. The kits are usually expensive along with the reagents
A- but become useful when large (not very large like 19200 but something like 200-300) patient samples to be tested?
200*145$ = 29000$ so then NGS way will be cheap and accurate?
R- Can't comment on this protocol. But looks interesting. However, the picture is blurred and I can't really understand wetlab much
Where is this cost coming from Avi?
Are we using biological replicates in this protocol. If yes how many??
A- 145$ is lowest cost from latest testing available afaik, and 300$ is for normal one that is used now. its approx and none of above info i have verified personally, but believe to be true
R- Depends Avi how deeply u sequence
only few parts you didn't get or many?
A- how deep we need to confirm if its no covid with high confidence? this can be extremely useful to mass screen communities in batches and seize them to prevent much harm to the economy and there are good ways coming ahead to do that part
R- Okay so they are using 2 amplicons
And 1 control
In total 3
I can't comment on that avi. Haven't had experience handling sequencing data of a virua
A- ok so this info came for 145$ test I had found - Update: After this story was published, the U.S. Food and Drug Administration issued a warning that at-home coronavirus tests are unauthorized. Read story here. https://www.statnews.com/2020/03/20/at-home-coronavirus-tests-are-here-should-you-take-them/
the at lab tests are arond 300 usd and in india around 10k afaik (govt.asked pvt.labs to do at 4500 let see how many does)
https://twitter.com/koeppelmann/status/1245037948099330048
B- The report suggests use of this kit the cost of which is following
A- R and i discussed same above. seems possible.
R- Yes Avinash. I think it's possible
We adopt such approach in NGS
R- I though doubt if the binary testing will actually save testing kits though
the grouping must be done carefully,Like all the samples coming from a single family will be a good idea In case if say out of 16 samples 8 tests positive
it will not help when we are testing symptomatic, or high risk, but can help if we mass testing and saving them who are negative from infection. like even a family or 2, unless whole family is high risk or having anyone symptomatic
R- Then if these guys are using some kind of index primers that are patient specific then this can really scale up testing
16/16 or 0/16 are ideal cases
